Protein Crystallography
 
 
Protein Crystallography
 

We are thrilled with the Rigaku X-ray generator and Saturn CCD detector combination in our X-ray Core Facility at the University of Puerto Rico.

Recently, we were able to collect 1.09 Å data on our Rigaku system that allowed us to see the conformational changes that take place in blackfin tuna myoglobin upon S-nitrosylation (Journal of Biological Chemistry, 282 (2007) 19773).

This post-translational protein modification is extremely sensitive to X-ray radiation, so we were not able to collect synchrotron data and had to use our home source. The sensitivity of the Saturn CCD detector allowed us to minimize the exposure times and still collect atomic resolution data that was necessary to deconvolute multiple conformations of the S-nitrosylated cysteine.

—Dr. Eric R. Schreiter
Director, X-ray Crystallography Core Facility
Protein Research and Development Center
University of Puerto Rico

 
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Macromolecular Crystallography Webinar training series

Welcome to the Rigaku Life Sciences Webinar Series. This free webinar series is designed to provide educational and training materials in X-ray crystallography methods. Each webinar will address a new topic ranging from optimization of crystallization conditions to solving the phase problem. We invite anyone to submit topic suggestions for future webinars and hope that you'll provide feedback for previous webinars. 

Next webinar:

  • Date/Time: Thursday, November 19, 2009 9:00 AM - 10:00 AM CST (15:00 GMT)
  • Title: Taking the edge off: The softer side of in-house SAD phasing
  • Presenters: Joe Ferrara & John Rose


Prof. John Rose

This webinar will cover Single wavelength Anomalous Diffraction enhanced by chromium radiation (CrSAD). In the first part, Dr. Ferrara will review the reasons for using CrSAD for de novo and phased molecular replacement structure solution, provide some examples and discuss the method. In the second half, Prof. Rose will work through some practical examples. Registrants will receive download information for sample data to review before the webinar. Prof. Rose will work through the sample cases as a part of the webinar.

To register for the webinar, please fill out this form.

For more information about Rigaku's Cr-Phasing HomeLab, visit this link.

Past webinars:

To view and/or download past webinars click here

To find information about future webinars, check this web site frequently. Upcoming webinars are also included in our monthly Life Sciences newsletter. If you would like to receive this monthly newsletter send email to info@rigaku.com.