Protein Crystallography
 
 
Saturn 944+
 
Rigaku is the leading provider of home lab­oratory X-ray equipment for protein and macro­molecular crystallo­graphy, including: fully integrated home lab systems, automated crystallization equipment, area detectors, X-ray generators and optics, software, as well as crystal cryo-cooling and automation robots.

A statistical analysis of X-ray crystal structures submitted to the Protein Data Bank (PDB) showed that structures solved with Rigaku home lab­oratory X-ray genera­tors comprised 17% of all submitted structures for 2005, the latest year of complete data. Of struc­ltures solved with home lab sources that year, 89% were done using Rigaku equipment.

Home lab PDB submissions by source vendor for 2005
89% of home lab PDB submissions for 2005 use Rigaku sources

The data conclusively demonstrate that Rigaku structural biology customers are collect­ively the most productive, far exceeding the nearest competitor in the home lab market.
 
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Saturn 944+
Saturn 944+
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Saturn 944+ CCD detector

Saturn 944+The new Saturn 944+, a 3rd generation CCD area X-ray detector designed and manufactured by Rigaku, now offers a further four-fold increase in readout speed. Superior dynamic range and high sensitivity is achieved through the use of transparent indium-tin-oxide (ITO) CCD sensor technology from Kodak®. Combining a large format CCD with an optimal fiber optic demagnification ratio, the Rigaku Saturn 944+ is the definitive area X-ray detector specifically optimized for the high throughput and spatial resolution needs of protein and macromolecular crystallography.

Perform rapid data collection 

Requirements of industrial structural biology today emphasize the need for high throughput in order to achieve an economically viable level of data generation. The Saturn 944+ CCD X-ray detector is the perfect tool for high throughput protein crystal screening. A high readout speed of 8 MHz delivers maximum data in minimal time.

Produce highest quality datasets

The full-frame CCD employed by the Saturn 944+ uses a proprietary 2-phase architecture and has high capacity pixels for extended dynamic range. To increase the sensitivity of the chip, one of the two polysilicon gates has been replaced with the transparent conductor indium-tin-oxide (ITO). Thus the design of the chip eliminates the need to resort to backside illumination and provides the intrinsically superior dark current characteristics needed to generate the highest quality data.

  • Advanced Kodak KAF-4320E CCD sensor
  • Superior front-illuminated ITO technology
  • Large 94 mm x 94 mm image area 2084 x 2084 pixel array with 4-port readout 
  • Approximately 15100:1 dynamic range
  • 18-bit analog-to-digital converter
  • Fast 2 MHz per port readout speed
  • Demagnification ratio of 1.88:1

Publish high-resolution structures 

With its high sensitivity, the Saturn 944+ is particularly powerful for the collection of weaker reflections, producing much higher resolution data. With a small point spread function, short crystal-to-detector distances are possible, thereby achieving high dataset completeness. With the available AFC11 or AFC Kappa goniometers, a flexible set of data acquisition strategies is supported to enhance coverage. Data collection is provided by StructureStudio™ and CrystalClear™, which includes d*TREK®. The Saturn 944+ is bundled with HKL-2000® for data processing. Final protein structure solution and refinement is supported by the optional MIFit+ software package that was developed in conjunction with the Automatic Structure Consortium.

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